fabrication of chitosan silk-based tracheal scaffold using freeze-casting method
نویسندگان
چکیده
background: since the treatments of long tracheal leisions are associated with some limitations, tissue enginered trachea is considered as an alternative option. this study aimed at preparing a composite scaffold, based on natural and synthetic materials for tracheal tissue engineering. methods: nine chitosan silk-based scaffolds were fabricated using three freezing rates (0.5, 1, and 2°c/min) and glutaraldehyde (ga) concentrations (0, 0.4, and 0.8 wt%). samples were characterized, and scaffolds having mechanical properties compatible with those of human trachea and proper biodegradability were selected for chondrocyte cell seeding and subsequent biological assessments. results: the pore sizes were highly influenced by the freezing rate and varied from 135.3×372.1 to 37.8×83.4 µm. swelling and biodegradability behaviors were more affected by ga rather than freezing rate. tensile strength raised from 120 kpa to 350 kpa by an increment of freezing rate and ga concentration. in addition, marked stiffening was demonstrated by increasing elastic modulus from 1.5 mpa to 12.2 mpa. samples having 1 and 2°c/min of freezing rate and 0.8 wt% ga concentration made a non-toxic, porous structure with tensile strength and elastic modulus in the range of human trachea, facilitating the chondrocyte proliferation. the results of 21-day cell culture indicated that glycosaminoglycans content was significantly higher for the rate of 2°c/min (12.04 µg/min) rather than the other (9.6 µg/min). conclusion: a homogenous porous structure was created by freeze drying. this allows the fabrication of a chitosan silk scaffold cross-linked by ga for cartilage tissue regeneration with application in tracheal regeneration.
منابع مشابه
Fabrication of Chitosan Silk-based Tracheal Scaffold Using Freeze-Casting Method
Background: Since the treatments of long tracheal lesions are associated with some limitations, tissue engineered trachea is considered as an alternative option. This study aimed at preparing a composite scaffold, based on natural and synthetic materials for tracheal tissue engineering. Methods: Nine chitosan silk-based scaffolds were fabricated using three freezing rates (0.5, 1, and 2°C/m...
متن کاملFabrication of Chitosan Silk-based Tracheal Scaffold Using Freeze-Casting Method
Background Since the treatments of long tracheal lesions are associated with some limitations, tissue engineered trachea is considered as an alternative option. This study aimed at preparing a composite scaffold, based on natural and synthetic materials for tracheal tissue engineering. Methods Nine chitosan silk-based scaffolds were fabricated using three freezing rates (0.5, 1, and 2°C/min) ...
متن کاملFabrication of Silk Scaffold Containing Simvastatin-Loaded Silk Fibroin Nanoparticles for Regenerating Bone Defects
Background: In the present study, a tissue engineered silk fibroin (SF) scaffold containing simvastatin-loaded silk fibroin nanoparticles (SFNPs) were used to stimulate the regeneration of the defected bone. Methods: At first, the porous SF scaffold was prepared using freeze-drying. Then simvastatin-loaded SFNPs were made by dissolvation method and embedded in the SF scaffold. Afterwards, the ...
متن کاملFabrication of HA/TCP scaffolds with a graded and porous structure using a camphene-based freeze-casting method.
A room temperature camphene-based freeze-casting method was used to fabricate hydroxyapatite/tricalcium phosphate (HA/TCP) ceramic scaffolds. By varying the solid loading of the mixture and the freezing temperature, a range of structures with different pore sizes and strength characteristics were achieved. The macropore size of the HA/TCP bioceramics was in the range of 100-200 microm, 40-80 mi...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian biomedical journalجلد ۲۱، شماره ۴، صفحات ۲۲۸-۲۳۹
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023